| 摘要 |
<p>The major allergenic protein LOl pI from pollen of ryegrass Lolium perenneL. is produced by recombinant DNA techniques. The DNA sequence encoding the above protein, expression vectors, host transformed and cell lines containing the coding sequence for LOl pI protein are also described. The use of the above DNA sequences and recombinant protein in nucleic hybridization, tissue specificity diagnosis and detection of specific antibodies in biological samples are also disclosed. The possible use of the promoter sequence of LOl pI in the developmental regulation of LOl pI gene expression or any other gene during the development of the pollen, in inhibiting pollen development or function and inducing nuclear male sterility are also disclosed.</p> |
