| 摘要 |
<p>Methods of stabilizing the three-dimensional conformations of proteins using "molecular sticks" to bridge and constrain tertiary protein structures are disclosed. These "molecular sticks" contain rigid multimeric spacer portions of various lengths, which are terminated on each end by chemical groups capable of covalent linkages to other designated chemical groups on different segments of the protein or peptide to be constrained. Proteins or peptides with stabilized tertiary structures are useful in diagnosis and therapy, especially under conditions where unstabilized proteins would be denatured.</p> |
