摘要 |
PURPOSE:To industrially and advantageously obtain the subject compound useful as a cosmetic, etc., by subjecting a specific microorganism capable of producing cellulose to seed culture, inoculating the seeds into a culture vessel of a bubble column, passing an oxygen-containing gas, culturing the microorganism, dividedly injecting the culture solution into a plate column culture vessel and stationarily culturing the solution. CONSTITUTION:A strain of Acetobactor aceti subsq. xylinum (ATCC 10821) is subjected to seed culture at 20-35 deg.C for 24-96hr while being shaken in a culture medium and the seeds are then inoculated into a culture medium in a bubble column culture vessel equipped with no stirrer and cultured therein at 20-35 deg.C for 24-120hr while ventilating oxygen-containing gas. The resultant culture solution is then dividedly injected into a plate column culture vessel and stationarily cultured at 20-35 deg.C for 72-600hr while ventilating the oxygen- containing gas to the surface of the culture solution. Thereby, the aimed microbi al cellulose is obtained. |