<p>Human hapatitis A virus (HAV) can be purified by a process in which master seed lots of HM-175 strain of hepatitis A virus are prepared from triply cloned virus by terminal dilution at passage level about 20 or preferably at least 20-30. The clones tested induced minimal or no hepatitis although significant antibody response was provoked in inoculated primates. A method of preparing the triply cloned inoculum is also described.</p>
申请公布号
CA1260392(A)
申请公布日期
1989.09.26
申请号
CA19850491066
申请日期
1985.09.18
申请人
UNITED STATES OF AMERICA, REPRESENTED BY THE SECRETARY, U.S. DEPA
发明人
DAEMER, RICHARD J.;FEINSTONE, STEPHEN M.;GUST, IAN D.;PURCELL, ROBERT H.