| 摘要 |
PURPOSE:To obtain high-purity EBV-related antigen by incorporating the gene coding EBV-related antigen into the restriction enzyme-nicked site in beta- galactosidase structural gene of lambda phage vector to form a manifestation vector. CONSTITUTION:Firstly, DNA fragment of EBV, e.g., DNA fragment containing the gene coding EA or VCA is obtained and connected with a linker. Second, the resultant fragment is incorporated into the restriction enzyme-nicked site in 1acZ gene of lambda phage vector and recovered as recombinant lambda phage through the in vitro packaging technique. Third, the product is assessed by a specified color-developing means and selected, from which DNA is extracted, and then DNA clone properly incorporated with the DNA of EBV is selected, thus obtaining the objective manifestation vector. Thence, Escherichia coli is made to be infected with the recombinant lambda phage and the bacteria inviable at high temperatures is selected to separate the lysogen for the recombinant lambda phage. |
