摘要 |
PURPOSE:To analyze an anion hard to elute with a good peak, by using a resin, which is based on a resin having a glycol type hydroxyl group and prepared by introducing a quaternary ammonium group into the primary hydroxyl group of said base resin, as a column packing agent. CONSTITUTION:The packing agent (hydrophylic resin) used in both of a precolumn 6 and a separation column 7 is obtained, for example, by using glyceryl methacrylate as a base material and introducing a quaternary ammonium group into the primary hydroxyl group thereof. This hydroxyl group is used in the introduction of an ion exchange group but partially left as a glycol type. When a boric acid-containing elute flows through the columns 6, 7, boric acid forms a complex ion along with the glycol type hydroxyl group to form a glycol borate ion on the surface of the resin to show ion exclusive effect. In this case, when the quaternary ammonium group (anion exchange group) is present simultaneously, the separation mode of ion exchange and ion exclusion can be controlled by controlling the addition amount of boric acid to the elute and a highly hydrophobic ion or an ion slow in elution can be rapidly eluted after all. |