| 摘要 |
<p>PURPOSE:To reduce operation for discarding a protein denaturing agent, by recovering the protein denaturing agent used in operation to denature, solubilize and renature insoluble foreign proteins from a solution containing the foreign proteins for reuse. CONSTITUTION:A protein denaturing agent, such as guanidine salt, urea or thiocyanate, is used to denature, solubilize and renature insoluble foreign proteins. In the process, the protein denaturing agent is separated and recovered from a solution in a stage of denaturing and solubilizing the produced insoluble foreign proteins or solution in a stage of renaturing by a means, e.g., dialysis, and as desired, proteins derived from a low-molecular host cell or other impurities are separated from the resultant solution containing the protein denaturing agent.</p> |
