Sorting bacteria according to aerobic characteristics for degradation of hydrocarbons, comprises defining general characteristics of selected bacteria, and determining a Gram stain test result, and isolating a bacteria

摘要

The method for sorting bacteria according to aerobic characteristics for degradation of hydrocarbons, comprises defining (1) general characteristics of selected bacteria, determining a Gram stain test result, isolating (4) a bacteria among other bacterias according to a first order of size of the selected bacteria, successively or concurrently subjecting the selected bacteria to oxygen and Gram stain test (5), and performing an initial identification such as marking and/or a first selection of bacterias according to the results of the behavioral analysis. The method for sorting bacteria according to aerobic characteristics for degradation of hydrocarbons, comprises defining (1) general characteristics of selected bacteria, determining a Gram stain test result, isolating (4) a bacteria among other bacterias according to a first order of size of the selected bacteria, successively or concurrently subjecting the selected bacteria to oxygen and Gram stain test (5), performing an initial identification such as marking and/or a first selection of bacterias according to the results of the behavioral analysis, performing a specific identification for each type of behavior responding to aerobic characteristics of bacteria, mixing bacteria with hydrocarbons to evoke a voluntary action of the selected bacteria and exposing the bacteria to Gram stain test, performing a second identification such as marking and/or second selection of bacterias, statistically analyzing marked bacterias to identify whether the selected bacteria rises above a predetermined threshold, and subjecting the selected or extracted bacteria to an operational phase. Other behavioral analysis of bacterias is produced by subjecting them to other oxygen and Gram stain test. The size of the bacteria is determined using the nature of the activities of individuals working in a specific field. The oxygen and Gram stain test are constituted by a reception of a targeted specific file comprising a questionnaire for collecting information. The targeted specific file is controlled by a communication system such as internet, telephone or fax. The questionnaire is transmitted and completed through internet. The marking comprises hybridization of a fluorescent probe. The bacteria is first extracted and then cultured.