| 摘要 |
PURPOSE:To obtain the titled enzyme useful for determining 5'-nucleotide in urine, blood serum, etc., or inosine 5'-monophosphate (IMP) content, etc., in a food and drink, etc., by cultivating a microorganism belonging to the genius Corynebacterium, Arthrobacter or Microbacterium in a culture medium. CONSTITUTION:A microorganism, such as Corynebacterium murisepticum No.7 (FERM-P No.206), Arthrobacter 11 (FERM-P No.207), Microbacterium No.205 (FERM-P No.4923) or Microbacterium No.205-MpCaGu-1 (FERM-P No.7102), in aerobically cultivated in a culture medium containing a carbon source, nitrogen source, inorganic phosphate, vitamins, etc., and 0.001-500mg/l cyclic-3',5'- nucleotide inhibitor added thereto to provide a cultivation filtrate. A 5'- nucleotidase, having the optimum pH 7.9-8.3 and capable of decomposing 5'-deoxyribonulceotide or 5'-ribonucleotide to form nucleosides and phosphoric acid is obtained from the above-mentioned resultant cultivation filtrate. |
