摘要 |
PURPOSE:To shorten an ester synthesis reaction time and obtain a high-quality modified fat or oil at a low cost, by using an immobilized lipase enzyme prepared by immobilizing lipase on an immobilizing carrier in the presence of an activity expressing agent. CONSTITUTION:An activity expressing agent consisting of various surfactants and polyol compounds in an amount of >=20%, preferably 50-500% based on lipase is used to immobilize the lipase on an adsorption type carrier or entrapping type carrier, etc., to provide an immobilized lipase agent. The moisture concentration of a raw material oil (fat and oil or a mixture thereof with a fatty acid and/or ester thereof) is adjusted to <=300ppm, preferably <=150ppm and the immobilized lipase in an amount of 2-30pts.wt. based on 100pts.wt. raw material oil is added thereto the carry out ester synthesis or interchange reaction at 30-90 deg.C for 1-24hr by batch reaction or continuous reaction using a column or fluidized tank, etc., and produce the aimed modified oil. |