摘要 |
PURPOSE:To prevent diffusion of a sample and solvent at the time of supplying a sample to a column and to obtain excellent sepn. performance by partitioning the inside of a cylinder which has a flow port for a mobile phase solvent on one end side and has a flow port of the sample on the other side. CONSTITUTION:The flow port 11 for the mobile phase solvent is provided to one end side of the cylinder 10 and the flow port 12 for the sample is provided to the other end side. A movable plug 9 which partitions the inside of the cylinder 10 to the flow port 11 side and the flow port 12 side is freely slidably provided in this cylinder 10. The movable plug 9 is first shifted toward the port 12 side of the cylinder 10 and the sample is injected from the port 12 into the cylinder 10 so that the sample is filled in the cylinder 10 while the plug 9 is pushed at the time of injecting the sample into the cylinder. The port 12 is then communicated with a column 16 and the mobile phase solvent is supplied from the port 11 into the cylinder 10, by which the plug 9 is pushed to the port 12 side and the sample in the cylinder 10 is pushed and is supplied into the column 16.
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