EPISOME VECTOR FOR DEVELOPING DNA FRAGMENT SELECTED FOR ENCODING POLYPEPTIDE IN CELL OF MAMMAL, RECOMBINANT VECTOR, MAMMAL HOST BODY AND PRODUCTION OF POLYPEPTIDE

摘要

The present invention is directed to an episomal expression vector with an efficient expression potential of cloned DNA sequences. The single vector plasmid of this invention joins DNA-fragments, which allow extra chromosomal replication of the vector in a wide selection of the host cells and fragments, which give the transfected cells resistance for a selection drug and a mammalian expression cassette comprising one of the strongest eucaryotic enhancers known at present. The vector also carries appropriate promoter and transcription processing signals. This new combination provides this expression vector with several properties that makes it optimal to be used in transfection of cell lines suitable for production and expression of foreign DNA sequences such as cDNA sequences joined into this vector with high efficiency.