Method of producing phospholipid derivatives.

摘要

Phospholipid derivatives having two phosphatidyl residues in the molecule - which derivatives except for diphosphatidylglycerol are new compounds - are produced from a starting material phospholipid derivative by transphosphatidylation. In this method, a phospholipid derivative (I) represented by the formula is reacted with a chain polyhydric alcohol represented by the formula in the presence of a phospholipase D having a transphosphatidylation activity (EC 3.1.4.4), thereby extracting a phospholipid derivative represented by the following formula from the reaction mixture In the formulas, R1 and R2 represent acyl groups or alkyl groups, R3 represents an alkylene group (proviso, hydrogen atom may be substituted with a hydroxyl group, an alkoxyl group, a hydroxyalkyl group or the like), and X represents the organic residue remaining after the removal of one hydroxyl group of a compound containing hydroxyl group.