NOVEL RECOMBINED PLASMID PBLAK1

摘要

PURPOSE:To make up a novel recombined plasmid pBLAK1 including a gene coding a fused protein from dihydrofolate reductase and bradykinin, and trans form E. coli with the plasmid to produce bradykinin. CONSTITUTION:The recombined plasmid pBLAK1 is stably copied in E. coli and gives the E. coli, the host, resistance to trimethoprim and ampicillin. The gene giving resistance to trimethoprim is recombined by modifying the 3'-termi nal of the dihydrofolate gene from Bacillus subtilis to code the fused protein from dihydrofolate reductase-bradykinin with 4474 pairs of bases. The recombinant plasmid pBLAK1 is introduced into E. coli C600 strain and the transformed strain is deposited as FERM P-9300.