| 摘要 |
<p>PURPOSE:To readily and rapidly measure Pseudouridine phi useful for clinical diagnosis of cancer, etc., by adding a specific antibody to a test specimen and measuring the amount of the formed antibody complex. CONSTITUTION:A mouse, etc., is immunized with an immunogen prepared by bonding pseudouridine phi (herein after referred to as phi) to bovine blood serum albumin, etc., as a carrier protein and splenic cells of the immunized mouse are then cloned to a monoclonal antibody (A) to the phi having the following properties. Class of antibody; IgG1. Antibody value; 64 times. Cross reactivity; 100% pseudouridine, 0% uridine, 7% uracil and 28% 5-fluorouracil. The resultant antibody (A) is then added to a test specimen, such as urine, and reacted at about 4 deg.C for about 1hr to afford a complex blend (B), which is then reacted with an alkaline phosphatase-labeled antibody to provide a reaction solution. A substrate solution containing p-nitrophenyl phosphate is added to the obtained reaction solution and reacted. A change in absorbance is subsequently measured to determined the amount of the phi in the test specimen.</p> |
