Method for mutagenesis by oligonucleotide-directed repair of a strand break.

摘要

A method for double-strand break repair and for site-directed mutagenesis in which a linear DNA molecule (target) and an oligonucleotide are cocultivated in a host cell. The oligonucleotide may have a mutation in its nucleotide sequence. The nucleotide sequence is, except for any mutation, homologous to that of the target and overlaps the nucleotide sequence at the ends of the target.