Enhanced large scale cultivation of bordetella pertussis cells for vaccine production using lactoglobulin

摘要

A Bordetella pertussis vaccine is prepared by deep tank cultivation using a seed grown in a biphasic culture system. An anion exchange resin in combination with beta -lactoglobulin and a Modified Cohen-Wheeler or Modified Stainer-Schlolte medium is used in the process resulting in enhancement of the growth, antigen production and potency of the phase I Bordetella pertussis vaccine. An acellular Bordetella pertussis vaccine derived from the culture broth and cells of Bordetella pertussis useful in preparing an acellular vaccine and which are grown in a medium containing beta -lactoglobulin and an anion exchange resin is also disclosed.