发明名称 |
cDNA clones coding for polypeptides exhibiting multi-lineage cellular growth factor activity |
摘要 |
Plasmid vectors are provided that carry complementary DNA (cDNA) clones coding for polypeptides exhibiting mammalian multi-lineage growth cell activity. One of these polypeptides is 166 amino acids in length, including a potential leader sequence of about 19 amino acids. The cDNA is derived from messenger RNA isolated from a mouse T-cell line after activation with concanavalin A. The cDNA was cloned by incorporation into a plasmid vector, which was then transformed into E. coli. The plasmid vector also contains DNA segments from the SV40 virus, permitting expression of the cDNA after transfection into a mammalian host cell, such as monkey COS-7 cells.
|
申请公布号 |
US4695542(A) |
申请公布日期 |
1987.09.22 |
申请号 |
US19840590867 |
申请日期 |
1984.03.19 |
申请人 |
DNAX RESEARCH INSTITUTE OF MOLECULAR AND CELLULAR BIOLOGY, INC. |
发明人 |
YOKOTA, TAKASHI;LEE, FRANK;RENNICK, DONNA;ARAI, KEN-ICHI |
分类号 |
A61K38/00;C07K14/52;(IPC1-7):C12P21/00;C07H15/12;C12N15/00;C12N1/00 |
主分类号 |
A61K38/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|