| 摘要 |
PURPOSE:To enable the mass-production of a phosphorus-containing compound SF-1293 substance useful as a herbicide in high yield at a low cost, by culturing a SF-1293 substance-producing strain belonging to Streptomyces genus in the presence of a specific compound. CONSTITUTION:Streptomyces hygroscopicus SF-1293 strain is inoculated in a medium containing glucose, peptone, CaCo3, etc., and cultured at 25-35 deg.C for 4-7 days e.g. by submerged technique while adding 0.1-20mg/ml of 4-(hydroxymethylphosphinyl)-2-oxobutyric acid of formula, its salt, monoester, diester or amide dividedly to the medium and, if necessary, adding dl-alanine to the medium at the same time. The obtained culture product is centrifuged and the supernatant liquid is purified to obtain the objective phosphorus- containing compound SF-1293 substance. |
