RAPID ANALYSIS OF ETHANOL IN BODY FLUIDS

摘要

Novel solid compositions are provided for assaying the levels of lower alcohols in body fluids. A carrier is impregnated with two or more enzymes, the first of which reduces a reducible agent in the presence of the alcohol. A chromogen is provided which reacts with either the reducer or the oxidized compound in the presence of a second enzyme or reagent, depending on the system, to produce a clinically significant indication of the alcohol present. A quantitative determination may be made by the provision of a competitive inhibitor to the first enzyme to provide a graduated response in colour according to the concentration of alcohol. Acetaldehyde trapping agents, buffers and enzyme stabilizers are provided as required.