Stabilization of enzymes useful in the production of glucosone and other enzymatic processes

摘要

Catalase and pyranose-2-oxidase are stabilized according to the invention by means of chemical treatment which render the enzymes resistant to thermal inactivation or inactivation of glucosone or both. Stabilized catalase crosslinked with diimido esters, such as dimethyl suberimidate and dimethyl adipimidate are claimed. The method for stabilizing catalase against thermal inactivation or glucosone inactivation or both comprising gradually adding a crosslinking agent to a catalase solution and maintaining pH and temperature control is claimed. Stabilized pyranose-2-oxidase amidinated with an amidinating agent such as ethyl acetimidate is claimed. A method for stabilizing catalase against thermal inactivation comprising gradually adding an amidinating agent to a pyranose-2-oxidase solution preferably with pH control is claimed. An improved process for producing glucosone from glucose using stabilized catalase or pyranose-2-oxidase or both according to the invention is also claimed.