Process for determining the presence of a specific IgE in human sera

摘要

The invention consists in a process for determining the presence of a specific IgE in human sera, characterised in that it comprises the following steps: binding of previously purified complex protein extracts upon a solid support, followed by incubation of the serum sample with the said bound protein extracts: washing non-specific proteins; incubation with anti-IgE monoclonal antibody labelled with <125>I or with an enzyme; subsequent washing of the solid support with the bound products and determination of the radioactivity or enzymatic activity incorporated into the solid support.