Hybridomas producing monoclonal antibodies specific for a human cell surface glycoprotein

摘要

Monoclonal antibodies are produced for a glycoprotein which is selectively expressed on the surface of human proliferating and immature hematopoietic cells but which is absent from normal peripheral blood cells. Mice are inoculated with human hematopoietic cells or fragments thereof, and spleen cells obtained from the mice are fused with mice myeloma cells to product hybridomas. The hybridomas are cultured as clones, and antibodies obtained from the individual clones are tested for their specificity for hematopoietic cells. Clones which produce antibodies specific for proliferating and immature hematopoietic cells are selected for further culturing to produce the antibody, and the antibody is obtained from the culture growth medium or from ascitic fluid of mice bearing the hybridoma tumor.