摘要 |
In a method of impregnating a tissue specimen with paraffin either directly after an optional treatment of the tissue specimen with a fixing agent or after the tissue specimen optionally has been treated with a fixing agent and/or a dehydrating agent an immersion of the tissue specimen into a bath of liquid paraffin takes place. The bath is kept below a predetermined temperature at which thermolabile components and properties of the tissue specimen undergo changes. The pressure of the bath is then lowered from atmospheric pressure to a predetermined pressure at which water present in the tissue specimen and/or, when appropriate, the dehydrating agent vaporizes, the pressure of the bath then being increased again to atmospheric pressure. |