发明名称 |
STABILIZATION OF EXTRACHROMOSOMAL GENE IN BACTERIA DURING CULTURE |
摘要 |
A novel method for stabilization of extra-chromosomal elements in bacteria during cultivation without use of antibiotics in the medium has been developed. The method comprises transformation of a host bacterium having a defect in a chromosomal gene needed for the synthesis or maintenance of the cell envelope with an extra-chromosomal element capable of suppressing the requirement caused by the chromosomal gene defect of the host bacterium. The invention furthermore provides for such transformed bacteria and stabilized extra-chromosomal elements and for a method for producing desirable products by inserting a gene for the desirable product into a stabilized extra-chromosomal element and cultivation of suitable host bacteria containing the stabilized extra-chromosomal element comprising the gene for the desirable product. It was demonstrated that according to the principles of this method, a plasmid including the dal gene of Bacillus subtilis and a gene encoding a desirable product can be maintained in an appropriate dal<->B. subtilis host in conventional media. The method thus allows for improved product yield and quality. |
申请公布号 |
JPS61139392(A) |
申请公布日期 |
1986.06.26 |
申请号 |
JP19850278850 |
申请日期 |
1985.12.11 |
申请人 |
NOVO IND AS |
发明人 |
BERUJIE KURAGU DEIDERIHISEN |
分类号 |
C07H21/04;C12N1/20;C12N1/21;C12N9/90;C12N15/00;C12N15/09;C12N15/67;C12N15/75;C12P21/02;C12R1/125;C12R1/19 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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