发明名称 |
Apparatus and method for cryopreparing biological tissue for ultrastructural analysis. |
摘要 |
<p>This invention relates to apparatus for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder at a temperature of less than - 140 DEG C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.</p> |
申请公布号 |
EP0184417(A2) |
申请公布日期 |
1986.06.11 |
申请号 |
EP19850308760 |
申请日期 |
1985.12.02 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
LINNER, JOHN G. |
分类号 |
G01N33/48;G01N1/42 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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