摘要 |
The invention relates to a method of preparing radionuclide-labelled proteins which are unstable at a pH lower than the physiological pH, in particular antibodies or antibody fragments, by reacting a protein which is provided with chelating group derived from Desferrioxamine, 8-hydroxyquinoline, imino triacetic acid-containing chelating agents, or imino diacetic acid-containing chelating agents, such as ethylenediamine tetracetic acid, diethylene triamine pentacetic acid or diaminocyclohexyl tetracetic acid, or salts hereof, or a chelating peptide or polypeptide such as transferrine, at a pH from 4 to 9, with a solution of an oxinate, a carboxylate, a dithiocarboxylate, an enolate, or a mixture thereof, of a radionuclide selected from the group consisting of radioactive indium, radioactive gallium, lead-203, ruthenium-97, mercury-197 and thallium(III)-201. The invention also relates to the labelled proteins thus obtained, to a radiopharmaceutical composition comprising said labelled proteins, to the use of said composition for performing a radiodiagnostic examination, and to a kit for performing such an examination. |