METHOD FOR FLUORESCENT MARKING OF POLYDEOXYRIBONUCLEOTIDE OR POLYRIBONUCLEOTIDE

摘要

PURPOSE:To enhance the detection sensitivity of marked polynucleotide by an optical marking method, by introducing an optical marking compound into the thiophosphoric acid group of a nucleic acid constitutional element. CONSTITUTION:The fluorescent marking of polynucleotide is performed by converting the phosphoric acid bond of polynucleotide to a thiophosphoric acid bond and reacting the thiol group of the thiophosphoric acid bond with a covalently bondable fluorescent marking compound specifically reacting said thiol group. The above-mentioned polynucleotide having the thiophosphoric acid bond can be synthesized by an org. synthesis method or enzymatic method. Especially, in the former org. synthesis method, it is easiest to introduce the thiophosphoric group into both terminal hydroxyl groups of polynucleotide and thiophosphoric acid, thiophosphoryl chloride or derivatives thereof may be reacted under a known condition.