| 摘要 |
PURPOSE:To recover a division sample in a short time by fixing a gel piece containing the division sample and providing a container made of a dialytic film around it, rotating the container, and allowing the division sample to migrate from gel to a solvent. CONSTITUTION:Electrodes 2 and 3 are provided on opposite surface of a fed tank 1. Further, the plane part of the gel piece which contains the division sample in the center is fixed by a support material 5 opposite the electrodes 2 and 3. Further, the container 4 made of the dialytic film s provided outside the support material 5 and rotated at a proper speed. Simultaneously, a DC current is flowed between both electrodes 2 and 3 then the division sample migrates from the gel and is dissolved in the solvent. At this time, the division sample stays in the container 4 because it dows not permeate the dialytic film. The container 4 is inverted in current direction to the dialytic film every time it is rotated by 180 deg. and film pores are prevented from being clogged. Therefore, the division sample is separated from the gel in a short time and the electric resistance of the film part is small, so heat generation is not caused. |
