Immunoassay for non-enzymatically glycosylated proteins

摘要

The proteins (I) and their fragments in a biological fluid are determined by contact of the fluid with anti (Amadori-rearranged glucose) (II) and determn. of the binding as a function of the amount of proteins or their fragments. Immunoassay reagent for determn. of non-enzymatically glucosylated proteins and protein fragments in a biological fluid comprises anti(Amadori-rearranged glucose) and a labelled reagent capable of binding with it and comprising a detectable label. - (I) and their fragments, e.g. polypeptides, peptides and aminoacids in biological fluids such as human serum or plasma can be determined selectively, esp. for providing an index of glycaemia. (0/2) EPAB- EP-111211 B An immunoassay method for determining nonenzymatically glucosylated proteins and protein fragments in a biological fluid, wherein the fluid is contacted with an antibody or antibody fragment and the binding of such proteins and protein fragments to such antibody reagent is determined as a function of the amount of such proteins and fragments thereof in the fluid, characteris-ed in that the antibody reagent has been raised against an immuno-gen comprising one or more residues of the formula (I), or conformers thereof, covalently linked to an immunogenic carrier material other than human hemoglobin. - (15pp) USAB- US4629692 A Antibody which sepecifically binds to a glucosylated peptide residue comprising Amadori-rearranged glucose of formula (I) is new. - In the formula, R is a bond, NH or a lysyl residue and the rearranged glucose moiety is coupled to its epsilon-amino gp. - An immunogen for preparing the antibody is also claimed.