| 摘要 |
PURPOSE:In the titled element having a layer containing a substrate of alkaline phosphatase on a support, to simplify analytical operation, by setting a developing layer containing a hydrophilic colloidal layer and an ultrafine substrate on the support. CONSTITUTION:A substrate of alkaline phosphatase is pulverized, and dispersed into a developing layer. A support is provided with a hydrophilic colloidal layer and the substrate-containing developing layer. Or, if necessary, another reagent layer and/or a reflective layer may be set on it. p-Nitrophenyl-phosphate or its salt is preferable as the substrate. A solution to be tested is dropped on the prepared analytical element, change with time of concentration of formed decomposition product is optically measured by specific spectrum while incubating it, to determine alkali phosphatase.
|
