发明名称 |
PROCESS FOR THE PRODUCTION OF AN IMMOBILIZED ENZYME SYSTEM AND THE PURIFICATION OF GLUCOSE ISOMERASE |
摘要 |
A composition comprising a thermally stable enzyme, for example intracellular or extracellular glucose isomerase, is purified by a method comprising heat treatment suitably at a temperature from 40 DEG C to 80 DEG C and removal of the solid matter formed. The resultant purified product, e.g. enzyme solution, is utilized to prepare an immobilized enzyme system which may be used to produce glucose from fructose. In a further embodiment a solution containing glucose isomerase is purified by acidification to precipitate proteinaceous solids, extraction of the solid with a buffer solution at pH 6 to 8, and salt fractionation of the extract solution. |
申请公布号 |
DE3071097(D1) |
申请公布日期 |
1985.10.24 |
申请号 |
DE19803071097 |
申请日期 |
1980.11.13 |
申请人 |
UOP INC. |
发明人 |
WINANS, VIDA;JACKSON, DENISE MARIE;TSUDA, YOSHIHISA |
分类号 |
C12N9/00;C12N9/92;C12N9/96;(IPC1-7):C12N11/00;C12N11/14 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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