摘要 |
An ion exchange method for the separation of hemoglobin A1 from its Schiff base precursors and from nonglycosylated hemoglobin in a sample of human blood. The known method of lysing the sample, using it to impregnate a weak cation exchange resin, eluting out the glycosylated components with a buffer solution containing from about 0.6 M to about 0.11 M alkali metal ion dissolved therein, and recovering the eluate, is modified by the inclusion of a dihydroxyboryl compound in either the hemolysate, the elution buffer, or both. |