| 摘要 |
<p>Construction of a DNA sequence for a messenger RNA encoding a polypeptide comprising a functional protein, or a bioactive part of it, is effected by (a) provision of a fragment of the DNA sequence encoding a C-terminal portion of the polypeptide (the fragment conforming in sequence to the natural sequence of this polypeptide); (b) provision of a fragment of the DNA sequence encoding the N-terminal portion of the poly-peptide; and (c) ligation of the 2 fragment (a) and (b) in proper reading frame relation to one another. Fragment (b) has its nucleotides sequentially arranged to provide on transcription messenger RNA that (1) properly encodes the respective portion of the amino acid sequence of the polypeptide and (2) demonstrates levels of conformational structure insufficient to interfere with its accessibility for efficient ribosomal translation. The DNA sequence is exclusive of the hybrid DNA sequence of human growth hormone (as dislosed in Nature 281,544 (1971)).</p> |
