MARKER FOR DISCRIMINATION OF FAGOPYRUM TATARICUM AND TRITICUM AESTIVUM USING CHLOROPLAST SEQUENCE AND DETECTING METHOD

摘要

The present invention relates to a marker to discriminate between Fagopyrum tartaricum and Triticum aestivum. Furthermore, according to the present invention, provided is a primer set to discriminate between Fagopyrum tartaricum and Triticum aestivum. In addition, the present invention relates to a method for discriminating between Fagopyrum tartaricum and Triticum aestivum, which comprises the following steps: isolating genomic DNA from a target sample; using the isolated genomic DNA as a template, and using the primer set to perform a polymerase chain reaction (PCR), thereby amplifying a target sequence; and identifying an amplified product. Furthermore, the present invention relates to a quantitative detection method of Fagopyrum tartaricum and Triticum aestivum. The method for discriminating between Fagopyrum tartaricum and Triticum aestivum allows people to check the varieties and mixing ratio of Fagopyrum tartaricum and Triticum aestivum which are included in a processed buckwheat product.