| 摘要 |
PURPOSE:To make a high-sensitive analysis of traces of saccharides with a simple apparatus by passing a soln. contg. saccharides through a liquid chromatographic column, reacting the separated saccharide soln. with a specific copper complex compd. and analyzing each saccharide with an electrochemical detector. CONSTITUTION:A mixed liquor of an elute from an elute tank 1 and a soln. contg. saccharides from a sample injecting valve 7 is sent into a gel permiation chromatographic column 8 using a filler for cation exchanges. A copper-bisphenanthroline complex or a copper-bipyridine complex (abbreviated as reaction reagent) is supplied froma storage tank 2 to the elute from the column 8, and mixed with the elute from the column 8 at a T-tube 9 to react in a reaction coil in a reaction tank 10 under a comparatively mild condition. The oxidation reduction potential from Cu<++11> <to> C<u+> is measured with a detector 12. The concn. of the reaction reagent ranging from 3.0-8.0mM is suitable for peak measurement. |
