| 摘要 |
PURPOSE:To magnify a measuring range to avoid the revelation frequency of antigen surplus phenomena and to minimize a diluting operation and to measure accurately and simply a high-concentration antigen by adding a free antibody to a reaction system together with the antibody sensitized to insoluble carrier particles. CONSTITUTION:An antibody sensitized to insoluble carrier particles, a free monospecific antibody capable of reacting with an antigen in a serum to be inspected, a normal serum of antibody producing animals and the serum to be inspected are allowed to react successively by shaking and mixing for several minutes in a liquid medium and said reaction product is diluted to a fixed volume by a diluent. Thereafter, light is irradiated to the flocculated liquid basing on the antigen-antibody reaction and the flocculating reaction is measured quantitatively by the variation of absorbance at this time. Fine particles of an organic polymer, individually dispersed coccus type bacteria such as staphylococcus, streptococcus etc., or inorganic oxides, mineral powder, metals are used for the insoluble carrier particles. Further, a buffer solution such as a physiological saline solution, phosphoric acid or tris-hydrochloric acid is used for the diluent. |
