PRODUCTION OF INTERFERON IN YEAST BY USE OF INVERTASE PROMOTER

摘要

A recombinant DNA segment which contains a SUC2 promoter linked to an interferon gene for directing the expression of the gene within a yeast cell. The expression of the gene for human leukocyte interferon is obtained in yeast and controlled by the SUC2 promoter of the yeast strain Saccharomyces cerevisiae. The SUC2 promoter is a DNA segment that contains the transcription start signal for the structural gene for invertase. The modified yeast strain of the invention displays a method of passive regulation of the expression of the interferon gene in yeast. Synthesis of the interferon is repressed when the yeast are grown in glucose medium, and derepressed when glucose is depleted.