| 摘要 |
NEW MATERIAL:An antitumor glycoproteinic substance having the following properties: White solid powder. Readily soluble in water and insoluble in organic solvents, e.g. methanol. Ampholytic electrolyte having an isoelectric point at 5.2pH. Positive to the biuret reaction, etc. and negative to the cysteine-sulfuric acid reaction. Average molecular weight: About 5000 (measured by the gel electrophoretic method). USE:An antitumor agent. PROCESS:A broth of a scallop (including a concentrate solution obtained in boiling or steaming the scallop) or a concentrate or a dried powder thereof is first dissolved in an aqueous solvent, and the undissolved substances are removed by the centrifugation or filtration, etc. The resultant aqueous solution is then desalted by the gel filtration or dialysis, and then subjected to the ion exchange chromatography with a basic ion exchanger. A component having an isoelectric point of 5.2pH is obtained from the resultant fraction, and then subjected to the fractionation treatment of molecular weight, e.g. vertical slab gel electrophoresis or gel filtration, etc. to afford the aimed antitumor glycoproteinic substance having 5000mol.wt. |
