摘要 |
PURPOSE:To increase easily the number of solid phase molecules per unit area of a solid phasing antibody without changing the surface area and shape of an insoluble solid phase carrier, by forming the composite body of the solid phasing antibody and antibody on the surface of said carrier. CONSTITUTION:In the case of using, for example, a glass beam, as an insoluble solid phase carrier, an antihuman IgE rabbit antibody and protein A are first brought into reaction to form a composite body of the antihuman IgE rabbit antibody-Protein A. A glass beam for converting the antihuman IgE rabbit antibody into a solid phase is put into the reacting liquid thereof and is brought into reaction to form the composite body of the antihuman IgE rabbit antibody- Protein A-antihuman IgE rabbit antibody on the surface of the glass beam, that is, the composite body of the solid phasing antibody-antibody. The increase in the number of solid phase molecules per unit area of the solid phasing antibody is thus made possible and the antihuman IgE rabbit antibody on the surface of the glass beam is amplified. |