PREPARATION OF L-PROLINE BY FERMENTATION

摘要

PURPOSE:To prepare L-proline in high efficiency, by culturing a microbial strain belonging to Serratia genus. CONSTITUTION:A microbial strain belonging to Serratia genus, resistant to purine-analog under high osmotic pressure, capable of producing L-proline, and preferably devoid of L-proline-decomposition enzyme, e.g. Serratia marcescens ODr-76 strain (FERM-P No.6552) which is an L-prolineoxidase-less L-proline- producing strain resistant to 3,4-dehydro-DL-proline in the presence of a high concentration sodium chloride, is innoculated in a nutrient medium and cultured under aerobic condition at 25-40 deg.C for 2-6 days. L-proline can be separated from the medium.