AUTOMATED METHOD FOR CELL VOLUME DETERMINATION

摘要

ORI-24 Automated Method For Cell Volume Determination Fluorescence volume exclusion signals are utilized to evaluate cell volume. Cells are suspended in a medium which is furnished with a fluorescent dye which neither penetrates nor adheres to the cells. Cells are analyzed by an optical flow cytometer, with illumination which causes the dye medium to fluoresce. Passage of a cell through the sensing zone therefore reduces the amount of fluorescent medium being illuminated, and proportionally reduces the fluorescence output signal. Fluorescence volume exclusion pulse height and area are related to cell volume.