发明名称 |
Method of immunoenzymatic assay utilizing DELTA 5,3-keto-steroid isomerase |
摘要 |
The invention relates to a novel enzymatic labelling agent for immunoenzymatic assays. The labelling agent is DELTA 5, 3-keto-steroid isomerase. The enzymatic activity bound to the antibody can be revealed advantageously by: (1) direct reading on the supernatent liquor or on the cake obtained by precipitation by means of a solvent, such as polyethylene glycol, with the use of soluble antibodies; (2) reading in solution after liberation of the enzyme from the antigen-antibody complex by a reducing agent, such as dithiotreitol; (3) immediate reading after filtration of a substrate solution on the insolubilized antibody-antigen-enzyme complex. The method can be used for immunoenzymatic assays automatically on substances present in very low concentrations, such as hypophysial hormones and plasmatic steroids.
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申请公布号 |
US4298686(A) |
申请公布日期 |
1981.11.03 |
申请号 |
US19790060677 |
申请日期 |
1979.07.25 |
申请人 |
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM) |
发明人 |
NICOLAS, JEAN C.;TEROUANNE, BEATRICE;DESCOMPS, BERNARD;DE PAULET, ANDRE C. |
分类号 |
G01N33/536;G01N33/535;G01N33/539;G01N33/58;G01N33/74;(IPC1-7):G01N33/54;C12N9/96 |
主分类号 |
G01N33/536 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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