GENERATION OF ANGIOTENSIN I FROM ANGIOTENSINOGEN BY TRIS(HYDROXYMETHYL)AMINOMETHANE AND MALEIC ACID BUFFER AND USE IN RADIOIMMUNOASSAY

摘要

In a radioimmunoassay for the determination of plasma renin activity, the use of a buffer solution of pH about 7.4 in the incubation of plasma samples, said buffer comprising about equimolar amounts of tris (hydroxymethyl) aminomethane and maleic acid, results in a high generation of angiotensin I. This process results in an improved testing prodcedure compared with the known art and it is useful in a radioimmunoassay for the determination of angiotensin I in a plasma sample.