| 摘要 |
An improved colorimetric aldolase method in which the quantity of aldolase in a sample is determined by the rate at which it enzymatically cleaves fructose-1, 6-diphosphate (FDP) into glyceraldehyde-3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP); the phosphate group is hydrolyzed from the GAP and DHAP; and one of the hydrolysis products is measured. The initial enzymatic reaction is carried out in a reaction mixture containing a concentrated tris-(hydroxymethyl)-aminomethane buffer, pH 7.0, and a small quantity of sodium fluoride, but without added hydrazine or other compound to prevent the enzymatic conversion of a great majority of GAP to DHAP in the reaction mixture. Hydrolysis of the reaction products and conversion to a colored reaction product is carried out by a procedure which yields intense, long-lasting, reproducible color. An improved standard includes dihydroxyacetone carried through the same procedure.
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