<p>D-Ribose is produced by cultivating a strain belonging to the genus Bacillus, which lacks sporulation ability or has high 2-deoxy-D-glucose-oxidizing activity or has both of these two properties and also lacks at least one of transketolase and D-ribulose phosphate 3-epimerase, to cause said strain to elaborate and accumulate a large amount of D-ribose. The thus accumulated D-ribose can be recovered in good yield.</p>