摘要 |
<p>METHOD OF BONDING ENZYMES TO POROUS INORGANIC CARRIERS Enzymes can be coupled che,mically to various water-insoluble inorganic carriers by reacting the carriers with 4,4'-bi(methoxybenzenediazonium chloride) to form a surface of diazo groups which are subsequently reacted with an enzyme solution to immobilize the enzymes. In preferred embodiments, the inorganic carriers are highly porous, have an average pore size of less than about 1000 .ANG., and consist of 4-200 mesh porous particles of materials selected from the group consisting of glass, silica, alumina, and mixtures of silica and alumina.</p> |