Cardiac glycoside enzyme conjugates

摘要

Enzyme conjugates are prepared of cardiac glycosides and aglycones for use in homogeneous enzyme immunoassays. The conjugates retain a substantial degree of the original activity of the enzyme and upon binding of receptor to the steroid moiety, a substantial diminution of enzyme activity is obtained. Various linking groups are employed for linking the steroid portion of the molecule to the enzyme, such as non-oxo carbonyl groups.