PROCESS FOR THE ENZYMATIC TREATMENT OF SUBSTRATES

摘要

A b s t r a c t For the enzymatic treatment of substrates, the substrate to be treated is brought into contact with a biocatalyser which was obtained by combining, in order to produce a fusion protein, a gene which codes for a biologically active substance and a DNA fragment which codes for a binding peptide which can interact with a carrier material then inserting the combined gene and DNA fragment into a suitable vector, transforming it in a suitable organism, culturing the organism, lysing the cells and bringing the lysate into contact with a carrier material which is capable of binding the binding peptide whereby the fusion protein binds to the carrier material by intermolecular interactions and the enzymatically treated substrate is subsequently isolated.