METHOD OF SCREENING DRUG ACTING ON CELL WALL

摘要

A method of screening a drug acting on cell wall which involves: (1) the step of incubating microorganisms, in which a reporter protein is fixed as GPI anchor protein respectively to the cell wall and the cell membrane, in the presence of a test substance; (2) the step of assaying the reporter protein released in the cultures of the above microorganisms; and (3) the step of judging the test substance as a drug having a selective inhibitory action on the cell wall, in case where the reporter protein is released into the culture of the microorganism having the reporter protein fixed to the cell wall but not substantially released into the culture of the other microorganism having the reporter protein fixed to the cell membrane.